Agilent Bio SEC

Agilent Bio SEC SemiPrep/Preparative LC Columns

Agilent Bio SEC offer fast, reliable, accurate performance for your biopharmaceutical analysis. They are easy to integrate into your workflow, and are available in a full range of pore sizes and 21.2 mm ID for preparative separations.

Agilent Bio SEC-3

These columns are a breakthrough technology for size exclusion chromatography (SEC). They are packed with spherical, narrowly dispersed 3 µm silica particles coated with a proprietary hydrophilic layer. This thin polymeric layer is chemically bonded to pure, mechanically stable silica under controlled conditions, ensuring a highly efficient size exclusion particle.

Available in 100 Å, 150 Å and 300 Å pore sizes to accommodate most peptide and protein size exclusion separations.

Agilent Bio SEC-5

These columns are packed with 5 µm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also promotes high pore volume, improving both peak capacity and resolution.

Available in 100 Å, 150 Å, 300 Å, 500 Å, 1000 Å and 2000 Å pore sizes to suit a variety of applications.

Agilent Bio SEC All pictures shown are for illustration purpose only. Actual product may vary.

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Features
  • Exceptional loading capacity, stability, and reproducibility for size-based biomolecule separations
  • Sharper peaks, higher resolution, and better protein recovery
  • Faster separations than large-particle SEC columns
  • Compatibility with most aqueous buffers
  • Excellent stability in high-salt and low-salt conditions
Specifications

Agilent Bio SEC-3

Pore Size Particle Size MW Range pH Range
100 Å 3 µm 100 - 100,000 2 - 8.5
150 Å 3 µm 500 - 150,000 2 - 8.5
300 Å 3 µm 5,000 - 1,250,000 2 - 8.5

 

Agilent BioSEC-5

Pore Size Particle Size MW Range pH Range
100 Å 5 µm 100 - 100,000 2 - 8.5
150 Å 5 µm 500 - 150,000 2 - 8.5
300 Å 5 µm 5,000 - 1,250,000 2 - 8.5
500 Å 5 µm 15,000 - 5,000,000 2 - 8.5
1000 Å 5 µm 50,000 - 7,500,000 2 - 8.5
2000 Å 5 µm >10,000,000 2 - 8.5
Literature